Preferential nuclear location of a transgene does not depend on its transcriptional activity during early mouse development.
Identifieur interne : 000330 ( France/Analysis ); précédent : 000329; suivant : 000331Preferential nuclear location of a transgene does not depend on its transcriptional activity during early mouse development.
Auteurs : E M Thompson [France] ; J P RenardSource :
- Chromosoma [ 0009-5915 ] ; 1998.
Descripteurs français
- KwdFr :
- Acides hydroxamiques (pharmacologie), Acétylation, Animaux, Blastocyste (physiologie), Développement embryonnaire (génétique), Embryon de mammifère (), Femelle, Grossesse, Histone (métabolisme), Mâle, Noyau de la cellule (génétique), Protéines de protozoaire (génétique), Protéines de protozoaire (métabolisme), Protéines du choc thermique HSP70 (génétique), Protéines du choc thermique HSP70 (métabolisme), Régulation de l'expression des gènes au cours du développement, Souris, Souris de lignée C57BL, Souris de lignée CBA, Souris transgéniques, Température élevée, Transcription génétique, Transgènes (génétique).
- MESH :
- génétique : Développement embryonnaire, Noyau de la cellule, Protéines de protozoaire, Protéines du choc thermique HSP70, Transgènes.
- métabolisme : Histone, Protéines de protozoaire, Protéines du choc thermique HSP70.
- pharmacologie : Acides hydroxamiques.
- physiologie : Blastocyste.
- Acétylation, Animaux, Embryon de mammifère, Femelle, Grossesse, Mâle, Régulation de l'expression des gènes au cours du développement, Souris, Souris de lignée C57BL, Souris de lignée CBA, Souris transgéniques, Température élevée, Transcription génétique.
English descriptors
- KwdEn :
- Acetylation, Animals, Blastocyst (physiology), Cell Nucleus (genetics), Embryo, Mammalian (drug effects), Embryonic Development (genetics), Female, Gene Expression Regulation, Developmental, HSP70 Heat-Shock Proteins (genetics), HSP70 Heat-Shock Proteins (metabolism), Histones (metabolism), Hot Temperature, Hydroxamic Acids (pharmacology), Male, Mice, Mice, Inbred C57BL, Mice, Inbred CBA, Mice, Transgenic, Pregnancy, Protozoan Proteins (genetics), Protozoan Proteins (metabolism), Transcription, Genetic, Transgenes (genetics).
- MESH :
- chemical , genetics : HSP70 Heat-Shock Proteins, Protozoan Proteins.
- drug effects : Embryo, Mammalian.
- genetics : Cell Nucleus, Embryonic Development, Transgenes.
- chemical , metabolism : HSP70 Heat-Shock Proteins, Histones, Protozoan Proteins.
- chemical , pharmacology : Hydroxamic Acids.
- physiology : Blastocyst.
- Acetylation, Animals, Female, Gene Expression Regulation, Developmental, Hot Temperature, Male, Mice, Mice, Inbred C57BL, Mice, Inbred CBA, Mice, Transgenic, Pregnancy, Transcription, Genetic.
Abstract
Changes in chromatin structure play an important role in regulation of the HSP70.1 gene during mouse preimplantation development. Using in situ PCR we have now examined whether the spatial organization of an HSP70.1 luciferase transgene within the nucleus is also a factor in regulating its expression. The transgene showed a preferential localization towards the nuclear periphery throughout preimplantation development. This preferential location was independent of the level of constitutive activity of the transgene and did not change when transgene expression was induced through core histone hyperacetylation at the eight-cell stage or by heat shock in blastocysts. In contrast, at the two-cell stage, when embryos are unable to continue development after heat shock, thermal stress provoked a significant disruption of the nuclear location of the transgene. These results do not agree with a recent model of embryonic genome activation in mice which hypothesizes that directed, active movement of DNA within the nucleus is a determinant factor in establishing early patterns of gene expression. Instead, they are consistent with models proposing that chromatin segments are restricted to nuclear subregions, but that they remain free to undergo substantial Brownian motion.
DOI: 10.1007/s004120050314
PubMed: 9880765
Affiliations:
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pubmed:9880765Le document en format XML
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<term>Animals</term>
<term>Blastocyst (physiology)</term>
<term>Cell Nucleus (genetics)</term>
<term>Embryo, Mammalian (drug effects)</term>
<term>Embryonic Development (genetics)</term>
<term>Female</term>
<term>Gene Expression Regulation, Developmental</term>
<term>HSP70 Heat-Shock Proteins (genetics)</term>
<term>HSP70 Heat-Shock Proteins (metabolism)</term>
<term>Histones (metabolism)</term>
<term>Hot Temperature</term>
<term>Hydroxamic Acids (pharmacology)</term>
<term>Male</term>
<term>Mice</term>
<term>Mice, Inbred C57BL</term>
<term>Mice, Inbred CBA</term>
<term>Mice, Transgenic</term>
<term>Pregnancy</term>
<term>Protozoan Proteins (genetics)</term>
<term>Protozoan Proteins (metabolism)</term>
<term>Transcription, Genetic</term>
<term>Transgenes (genetics)</term>
</keywords>
<keywords scheme="KwdFr" xml:lang="fr"><term>Acides hydroxamiques (pharmacologie)</term>
<term>Acétylation</term>
<term>Animaux</term>
<term>Blastocyste (physiologie)</term>
<term>Développement embryonnaire (génétique)</term>
<term>Embryon de mammifère ()</term>
<term>Femelle</term>
<term>Grossesse</term>
<term>Histone (métabolisme)</term>
<term>Mâle</term>
<term>Noyau de la cellule (génétique)</term>
<term>Protéines de protozoaire (génétique)</term>
<term>Protéines de protozoaire (métabolisme)</term>
<term>Protéines du choc thermique HSP70 (génétique)</term>
<term>Protéines du choc thermique HSP70 (métabolisme)</term>
<term>Régulation de l'expression des gènes au cours du développement</term>
<term>Souris</term>
<term>Souris de lignée C57BL</term>
<term>Souris de lignée CBA</term>
<term>Souris transgéniques</term>
<term>Température élevée</term>
<term>Transcription génétique</term>
<term>Transgènes (génétique)</term>
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<term>Protozoan Proteins</term>
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<keywords scheme="MESH" qualifier="drug effects" xml:lang="en"><term>Embryo, Mammalian</term>
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<term>Embryonic Development</term>
<term>Transgenes</term>
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<term>Protéines de protozoaire</term>
<term>Protéines du choc thermique HSP70</term>
<term>Transgènes</term>
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<term>Histones</term>
<term>Protozoan Proteins</term>
</keywords>
<keywords scheme="MESH" qualifier="métabolisme" xml:lang="fr"><term>Histone</term>
<term>Protéines de protozoaire</term>
<term>Protéines du choc thermique HSP70</term>
</keywords>
<keywords scheme="MESH" qualifier="pharmacologie" xml:lang="fr"><term>Acides hydroxamiques</term>
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<term>Animals</term>
<term>Female</term>
<term>Gene Expression Regulation, Developmental</term>
<term>Hot Temperature</term>
<term>Male</term>
<term>Mice</term>
<term>Mice, Inbred C57BL</term>
<term>Mice, Inbred CBA</term>
<term>Mice, Transgenic</term>
<term>Pregnancy</term>
<term>Transcription, Genetic</term>
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<term>Animaux</term>
<term>Embryon de mammifère</term>
<term>Femelle</term>
<term>Grossesse</term>
<term>Mâle</term>
<term>Régulation de l'expression des gènes au cours du développement</term>
<term>Souris</term>
<term>Souris de lignée C57BL</term>
<term>Souris de lignée CBA</term>
<term>Souris transgéniques</term>
<term>Température élevée</term>
<term>Transcription génétique</term>
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<front><div type="abstract" xml:lang="en">Changes in chromatin structure play an important role in regulation of the HSP70.1 gene during mouse preimplantation development. Using in situ PCR we have now examined whether the spatial organization of an HSP70.1 luciferase transgene within the nucleus is also a factor in regulating its expression. The transgene showed a preferential localization towards the nuclear periphery throughout preimplantation development. This preferential location was independent of the level of constitutive activity of the transgene and did not change when transgene expression was induced through core histone hyperacetylation at the eight-cell stage or by heat shock in blastocysts. In contrast, at the two-cell stage, when embryos are unable to continue development after heat shock, thermal stress provoked a significant disruption of the nuclear location of the transgene. These results do not agree with a recent model of embryonic genome activation in mice which hypothesizes that directed, active movement of DNA within the nucleus is a determinant factor in establishing early patterns of gene expression. Instead, they are consistent with models proposing that chromatin segments are restricted to nuclear subregions, but that they remain free to undergo substantial Brownian motion.</div>
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